EVIDENCE FOR PENETRANT AND NON-PENETRANT THIOL REAGENTS AND THEIR USE IN THE LOCATION OF RAT LIVER MITOCHONDRIAL D(-)$-HYDROXYBUTYRATE DEHYDROGENASE” Y. GAUDEMER and N. LATRUFFE
نویسنده
چکیده
The implication of mitochondrial protein thiols has been postulated in several functions: coupling mechanism, transport, conformations changes, dehydrogenases, etc.. A general review concerning these topics has been published recently [ 11. In order to localize these SH-groups it is necessary to evaluate the penetrability of SH reagents through the inner membrane; this was done by measuring, in the presence of different thiol reagents in isotonic conditions and varying the experimental conditions, the amount of free reduced (GSH) and oxidized (GSSG) glutathione in the matrix compartment. These results put forward two types of reagents: penetrant and non-penetrant thiol reagents. The use of these two types permits to localize the rat liver mitochondrial D(-)-fl-hydroxybutyrate dehydrogenase on the inside part of the inner membrane. Non penetrant thiol reagents do not inhibit the D(-)-/_%hydroxybutyrate oxidation in intact mitochondria but only in sonicated mitochondria whereas penetrant thiol reagents inhibit immediately with intact mitochondria. This location is confirmed by
منابع مشابه
Molecular and cellular biology of helminth parasites IV.
FOR ORAL PRESENTATIONS Nematode genomics and informatics: leveraging C. elegans PAUL W. STERNBERG. CALIFORNIA INSTITUTE OF TECHNOLOGY, PASADENA, CA HHMI, Caltech and WormBase C. elegans has emerged as a model organism, not only for classical biology but also for genomics and bioinformatics. With the emergence of additional genome informatic resources, the C. elegans and parasitic helminth commu...
متن کاملGains from diversification on convex combinations: A majorization and stochastic dominance approach
By incorporating both majorization theory and stochastic dominance theory, this paper presents a general theory and a unifying framework for determining the diversification preferences of risk-averse investors and conditions under which they would unanimously judge a particular asset to be superior. In particular, we develop a theory for comparing the preferences of different convex combination...
متن کاملImproved immunogenicity of tetanus toxoid by Brucella abortus S19 LPS adjuvant.
BACKGROUND Adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. Despite immunostimulatory properties, the use of bacterial lipopolysaccharide (LPS) as an adjuvant has been hampered due to its toxicity and pyrogenicity. Brucella abortus LPS is less toxic and has no pyrogenic properties compared to LPS from other gram negati...
متن کاملSteady electrodiffusion in hydrogel-colloid composites: macroscale properties from microscale electrokinetics.
A rigorous microscale electrokinetic model for hydrogel-colloid composites is adopted to compute macroscale profiles of electrolyte concentration, electrostatic potential, and hydrostatic pressure across membranes that separate electrolytes with different concentrations. The membranes are uncharged polymeric hydrogels in which charged spherical colloidal particles are immobilized and randomly d...
متن کاملPerturbative Analysis of Dynamical Localisation
In this paper we extend previous results on convergent perturbative solutions of the Schrödinger equation of a class of periodically timedependent two-level systems. The situation treated here is particularly suited for the investigation of two-level systems exhibiting the phenomenon of (approximate) dynamical localisation. We also present a convergent perturbative expansion for the secular fre...
متن کامل